Work

A Simple and Sensitive Polymerase Chain Reaction Method for the Detection of Food-Related Bacteria.

Year: 2000

Type: article

Abstract: We devised a simple and efficient method of preparing chromosomal DNA from Gram-positive bacteria for use as a template in PCR, which consists of achromopeptidase treatment followed by Chelex 100 treatment. Using template DNA prepared by this method, it was possible to detect as few as one colony forming unit of some kinds of food-related bacteria using universal primers targeting the bacterial 16S ribosomal RNA gene or specific primers for bacterial genes under fixed thermocycling conditions.

Source: Food Science and Technology Research

DOI: 10.3136/fstr.6.115

Authors: Koji NAGASHIMA, Takeshi SHIMIZU, Kouichi TAKESHI, Makoto KAWAKAMI, Daisuke YASOKAWA, Ryoji NAKAGAWA, Yukihiro OKUMURA

Institution: Hokkaido Institute of Public Health

Language: English

Topic: Bacterial Identification and Susceptibility Testing

Subfield: Microbiology (medical)

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

OpenAlex ID: W2053166995

FWCI: 0.172

Cites: 14

Cited by: 9

Related to: 10