Work

FolX from Pseudomonas aeruginosa is octameric in both crystal and solution

Year: 2012

Type: article

Abstract: FolX encodes an epimerase that forms one step of the tetrahydrofolate biosynthetic pathway, which is of interest as it is an established target for important drugs. Here we report the crystal structure of FolX from the bacterial opportunistic pathogen Pseudomonas aeruginosa, as well as a detailed analysis of the protein in solution, using analytical ultracentrifugation (AUC) and small-angle X-ray scattering (SAXS). In combination, these techniques confirm that the protein is an octamer both in the crystal structure, and in solution.

Source: FEBS Letters

DOI: 10.1016/j.febslet.2012.03.031

Authors: Mads Gabrielsen, Katherine S.H. Beckham, Richard J. Cogdell, Olwyn Byron, Andrew J. Roe

Institution: University of Glasgow

Language: English

Topic: Enzyme Structure and Function

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,980