Work

DNA sequencing by single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA

Year: 1993

Type: conference-paper

Abstract: We are developing a laser-based technique for the rapid sequencing of large DNA fragments (several kb in size) at a rate of 100 to 1000 bases per second. Our approach relies on fluorescent labeling of the bases in a single fragment of DNA, attachment of this labeled DNA fragment to a support, movement of the supported DNA into a flowing sample stream, sequential cleavage of the end nucleotide from the DNA fragment with an exonuclease, and detection of the individual fluorescently labeled bases by laser-induced fluorescence.

Source: Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE

DOI: 10.1117/12.146712

Authors: Peter M. Goodwin, Jay A. Schecker, Charles W. Wilkerson, Jr., Mark L. Hammond, W. Patrick Ambrose, James H. Jett, John C. Martin, Babetta L. Marrone, Richard A. Keller, Alberto Haces, Po-Jen Shih, John D. Harding

Institution: Los Alamos National Laboratory

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W2058190876

FWCI: 5.65

Cites:

Cited by: 6

Related to: 10