The Fluorimetric Microdetermination of Glyoxylic Acid in Blood, Urine and Bacterial Extracts
Year: 1965
Type: article
Abstract: 1. A spectrophotofluorimetric method for the determination of glyoxylic acid in biological materials is described. 2. The method is based on the reaction between glyoxylic acid and resorcinol in acid solution, a fluorescent complex being obtained on the subsequent addition of alkali. 3. The reaction was found to be sensitive and highly specific, the minimum detectable amount of glyoxylic acid being 1.35x10(-8) mole. 4. The urinary excretion of glyoxylic acid by ten normal adults ranged from 1.4 to 4.7mg./24hr. Small but measurable amounts of glyoxylic acid were found in cell-free extracts of Pseudomonas oxalaticus OX1 grown on oxalic acid as a source of carbon. No glyoxylic acid was detected in human serum.
Source: Biochemical Journal
DOI: 10.1042/bj0960218
Authors: PM ZAREMBSKI, A HODGKINSON
Institution: Leeds General Infirmary
Language: English
Subfield: Urology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Funder: University of Leeds
OpenAlex ID: W206385292