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Quantitative Detection of Listeria monocytogenes in Biofilms by Real-Time PCR

Year: 2005

Type: article

Abstract: A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 10(2) CFU/cm2.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.71.4.2190-2194.2005

Authors: Morgan Guilbaud, Pierre de Coppet, Fabrice Bourion, Cinta Rachman, Hervé Prévost, Xavier Dousset

Institutions: École Nationale d'Ingénieurs des Techniques des Industries Agro-Alimentaires, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Language: English

Topic: Listeria monocytogenes in Food Safety

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2064976151

FWCI: 6.273

Cites: 23

Cited by: 62

Related to: 10