Synthesis of a 37-residue peptide amide corresponding to the entire amino acid sequence of .ALPHA.-form of rat calcitonin gene-related peptide (.ALPHA.-rCGRP).
Year: 1987
Type: article
Abstract: The heptatriacontapeptide amide corresponding to the entire amino acid sequence of α-form of rat calcitonin gene-related peptide (α-rCGRP) was synthesized by the conventional solution method. All protecting groups employed were removed by treatment with 1 M trifluoromethanesulfonic acid-thioanisole-trifluoroacetic acid, and the deprotected peptide was subjected to airoxidation to form the intramolecular disulfide bond. After purification by gel-filtration on Sephadex G-50, followed by reversed-phase high performance liquid chromatography, a highly purified sample of synthetic α-rCGRP was obtained. In terms of suppression of bone 45Ca-release stimulated by synthetic human parathyroid hormone (1-34), synthetic α-rCGRP was as active as synthetic human CGRP.
DOI: 10.1248/cpb.35.60
Authors: KENSHI ANDO, KOUKI KITAGAWA, SHINYA KIYAMA, TATSUHIKO KAWAMOTO, TADASHI AKITA, ITSUO YAMAMOTO, KANJI TORIZUKA
Institutions: Tokushima University, Kyoto University
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2065246741