3'-azido- 3'-deoxythymidine(AZT)投与ラットにおける酸化ストレスならびに心機能低下に対するタマネギとイチョウ葉エキスの効果
Year: 2005
Type: article
Abstract: While 3'-azido-3'-deoxythymidine (AZT) is being used as a therapeutic agent for AIDS, it induces oxidative stress in the heart and reduces cardiac function. The present study thus examined whether reduced cardiac function associated with AZT administration can be ameliorated by ingesting antioxidant foods such as onion and Ginkgo biloba extract. In order to facilitate induction of oxidative stress, male Fischer-F344 rats were given a high fat (12%) diet (HF) from 2- to 12-months-old. A HF including either 0.5% lyophilized onion powder (HF+O) or 0.2% Ginkgo biloba extract (HF+G) was then given to the rats. Furthermore, from 17.5-months-old, AZT 40 mg/kg/day was administered for 2 weeks. Electrocardiography (ECG) was performed 3 days after the final administration. The rats were euthanized and levels of cardiac lipid peroxide (LPO) and glutathione were measured. Serum lipid components, which are closely related to cardiac function, were measured at the same time. The results were as follows: 1) ECG showed no significant difference between diet groups, although cardiac function tended to be lower for rats with AZT than for rats without. 2) Levels of cardiac LPO and glutathione for the HF group were unchanged by AZT administration. However, glutathione levels were significantly higher for the HF+O group with AZT than for the HF+O group without AZT, suggesting that onion suppresses oxidative stress. 3) Levels of plasma total cholesterol (T-Ch), HDL-cholesterol (HDL-Ch), LDL-cholesterol (LDL-Ch) and lipid peroxide (LPO) were significantly higher for the HF group with AZT than for the HF group without AZT. However, compared to the HF group with AZT, T-Ch, LDL-Ch and Atherogenic Index (AI) were lower for the HF+G group with AZT. These findings suggest that onion is effective against cardiac oxidative stress, and Ginkgo biloba extract is effective in preventing changes to serum lipid components associated with AZT.
DOI: 10.24552/00001639
Language: Japanese
Subfield: Biochemistry (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W206557394