Year: 1997
Type: article
Abstract: Several tests were carrled out to enable evaluation of the precis~on with which sandy sediment bacteria could be enumerated.This represented the first attempt to place direct epifluorescence counts of benthic bacteria on a quantitative, rather than relative, ground.The tests cornb~ned in situ radioisotope ( [ ' H ] , [14C]) labeling of sediment bactena, bacterial dislodgment by ultrasonic treatment, and bacterial enumeration via fluorescent staining.The results provided direct and indirect evidence that the employed protocol for bacterial enumeration accounted for 88 to 98% of all bacteria present in sediments.The identified approach thus allowed for a rather complete quantification of sediment bacteria.Since this protocol was more effective that the alternative techniques used in earlier studies, sediment bacteria may have been repeatedly undercounted in the past.
Source: Marine Ecology Progress Series
DOI: 10.3354/meps151011
Authors: SS Epstein, D Alexander, K Cosman, A Dompé, S Gallagher, J Jarsobski, E Laning, R Martinez, G Panasik, C Peluso, R Runde, E Timmer
Institutions: Northeastern University, Marine Biological Laboratory
Language: English
Subfield: Ecology
Field: Environmental Science
Domain: Physical Sciences
Open Access status: bronze
Funder: National Science Foundation
OpenAlex ID: W2067332467