Clinical variability and novel mutations in the NHEJ1 gene in patients with a Nijmegen breakage syndrome-like phenotype
Year: 2010
Type: article
Study design: Case Report
Abstract: We have previously shown that mutations in the genes encoding DNA Ligase IV (LIGIV) and RAD50, involved in DNA repair by nonhomologous-end joining (NHEJ) and homologous recombination, respectively, lead to clinical and cellular features similar to those of Nijmegen Breakage Syndrome (NBS). Very recently, a new member of the NHEJ repair pathway, NHEJ1, was discovered, and mutations in patients with features resembling NBS were described. Here we report on five patients from four families of different ethnic origin with the NBS-like phenotype. Sequence analysis of the NHEJ1 gene in a patient of Spanish and in a patient of Turkish origin identified homozygous, previously reported mutations, c.168C>G (p.Arg57Gly) and c.532C>T (p.Arg178Ter), respectively. Two novel, paternally inherited truncating mutations, c.495dupA (p.Asp166ArgfsTer20) and c.526C>T (p.Arg176Ter) and two novel, maternal genomic deletions of 1.9 and 6.9 kb of the NHEJ1 gene, were found in a compound heterozygous state in two siblings of German origin and in one Malaysian patient, respectively. Our findings confirm that patients with NBS-like phenotypes may have mutations in the NHEJ1 gene including multiexon deletions, and show that considerable clinical variability could be observed even within the same family.
Source: Human Mutation
DOI: 10.1002/humu.21315
Authors: Véronique Dutrannoy, Ilja Demuth, Ulrich Baumann, Detlev Schindler, Kateryna Konrat, Heidemarie Neitzel, Gabriele Gillessen-Kaesbach, Janina Radszewski, Susanne Rothe, Mario T. Schellenberger, Gudrun Nürnberg, Peter Nürnberg, Keng Wee Teik, Revathy Nallusamy, André Reis, Karl Sperling, Martin Digweed, Raymonda Varon
Institutions: Charité – Universitätsmedizin Berlin, Medizinische Hochschule Hannover, University of Würzburg, University of Lübeck, University of Cologne, Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases, Hospital Pulau Pinang, Friedrich-Alexander-Universität Erlangen-Nürnberg, Universitätsklinikum Erlangen
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2073299702