Work

Protein Crystallization by Combining Laser Irradiation and Solution-Stirring Techniques

Year: 2005

Type: article

Abstract: Bovine adenosine deaminase in the absence of an inhibitor (free-ADA) does not form crystals when using conventional crystallization methods. Using a solution-stirring technique, we recently succeeded in generating a small number of free-ADA crystals. In this paper, we demonstrate the combination of laser-irradiated growth and stirring (COLAS). This technique was found to be useful for controlling crystal nucleation and growth, which led to the production of a much larger number of high-quality free-ADA crystals.

Source: Japanese Journal of Applied Physics

DOI: 10.1143/jjap.44.1365

Authors: Hiroaki Adachi, Ai Niino, Satoshi Murakami, Kazufumi Takano, Hiroyoshi Matsumura, Takayoshi Kinoshita, Masaichi Warizaya, Tsuyoshi Inoue, Yusuke Mori, Takatomo Sasaki

Institutions: The University of Osaka, Japan Science and Technology Agency, The Institute of Scientific and Industrial Research, The University of Osaka

Language: English

Topic: Crystallization and Solubility Studies

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: bronze

Funders: Ministry of Education, Culture, Sports, Science and Technology, Research Foundation for Opto-Science and Technology

OpenAlex ID: W2076957868

FWCI: 0.813

Cites: 14

Cited by: 9

Related to: 10