Work

Distribution of Saccharide Residues on Membrane Fragments from a Myeloma-Cell Homogenate: Its Implications for Membrane Biogenesis

Year: 1972

Type: article

Abstract: Ferritin conjugates of two plant agglutinins, concanavalin A and ricin, have been used as specific electron microscopic stains for covalently-bound saccharide residues on membrane fragments from a myeloma-cell homogenate. The results indicate that different saccharide residues are uniformly localized to a single surface of each membrane fragment. In particular, the ferritin-concanavalin A conjugate binds exclusively to the cisternal side of membrane fragments of the rough endoplasmic reticulum. If it is postulated that the biogenesis of eukaryotic plasma membranes involves an assembly-line process from precursor intracellular membranes, these observed asymmetric distributions of saccharides on cell membranes can be explained.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.69.10.2945

Authors: Hiroshi Hirano, Brenda Parkhouse, Garth L. Nicolson, Edwin S. Lennox, S. J. Singer

Institutions: Salk Institute for Biological Studies, University of California San Diego

Language: English

Topic: Cellular transport and secretion

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2076973467

FWCI: 9.286

Cites: 25

Cited by: 182

Related to: 10