Complement Activation by Stratum corneum Through the Antibody-Independent Alternative Pathway
Year: 2009
Type: article
Abstract: KevWords. Stratum corneum • Alternative complement pathway • C5a anaphylatoxinWe provide evidence that stratum corneum (SC) acti vates complement through the alternative pathway to generate C5a anaphylatoxin.By immunofluorescence studies it was shown that in addition to circulating IgG autoantibodies, there were anti-SC antibodies of IgM and IgA classes in the sera from normal individuals.However, all the titers were significantly lower than the level of C3 deposition between corneocytes.By contrast, there occurred no C lq deposition.Immunoelectrophoretically the orthokeratotic SC homogenates were found to induce the conversion of C3 from native C3 to C3b in fresh human serum even when the classic pathway was blocked by Ca2+-chilation.Enzyme immunoassay showed that factor B split product.Bb. was generated by the SC homogenate in the Ca2+-chilated serum.Radioimmunoassay for C5a also demon strated that the SC homogenates could generate C5a ana phylatoxin in serum to an extent similar to that in nontreated serum when restricted to the alternative pathway activation; neutrophil chemotactic activity was generated in Ca2+-chilated serum at levels comparable to that generated in nontreated fresh serum.We separated the SC samples into a comified envelope, and soluble and keratin fractions.The cornified envelope was more effective in
Source: Dermatologica
DOI: 10.1159/000248466
Institution: Tohoku University
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2080124971