Work

The Studies on the Transfection of EGFP-Fused Porcine Circovirus Type 2 Genome

Year: 2011

Type: article

Abstract: This study described construction and transfection of an EGFP-fused Porcine Circovirus Type 2 (PCV2) genome and the recovery of the virus. Posttransfection, PCV2 (ORF1)-EGFP/pSK, PCV2 (ORF3)-EGFP/pSK, PCV2 (ORF4)-EGFP/pSK and PCV2(ORF5)-EGFP/pSK showed no fluorescent signals in transfected cells, while green fluorescent signals were observed in the nuclei of PK-15 cells after PCV2 (ORF2)-EGFP/pSK transfection. The presence of ORF2-EGFP fusion protein was demonstrated by dual signals of green fluorescence and anti-PCV2 antibodies conjugated with rhodamine in an immunofluorescence assay (IFA). Furthermore, the released EGFP-fused PCV2 genome was demonstrated by real-time PCR.

Source: Journal of Veterinary Medical Science

DOI: 10.1292/jvms.10-0490

Authors: Hsu-Chung Gabriel CHEN, Shu-Hsiang YANG, Tsun-Yung KUO, Shiow-Suey LAI

Institutions: National Taiwan University, National Ilan University

Language: English

Topic: Animal Virus Infections Studies

Subfield: Animal Science and Zoology

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: hybrid

OpenAlex ID: W2083974232

FWCI: 0.5

Cites: 10

Cited by: 1

Related to: 10