Work

Hydrogen peroxide-induced enhancement of prostanoid release and myocardial damage by its washout in isolated rat heart

Year: 1999

Type: article

Abstract: Hydrogen peroxide (H2O2) contributes to the development of reperfusion injury. The aim of this study was to characterize the effects of H2O2 on cardiac function, autacoid release and myocardial damage. Rat hearts were Langendorff-perfused with Krebs solution containing 70, 200 or 700 μM H2O2 for 30 min, then washed out with normal Krebs solution. The left ventricular pressure, heart rate and coronary perfusion pressure were monitored, released prostanoid and NO was quantified and the activity of released glutamic oxaloacetic transaminase (GOT) was estimated. H2O2 (70 μM) significantly increased prostacyclin release with no evidence of myocardial damage. H2O2 (200 μM) stimulated the release of all the prostanoids quantified markedly without affecting NO release. GOT release did not increase during H2O2 perfusion, but did increase after washout. Inhibition of prostanoid release with indomethacin did not affect the washout-induced increase in GOT release. These results indicate that a low concentration of H2O2 stimulates prostanoid release without affecting myocardial contraction, while higher concentrations damage the heart indirectly. The mechanism of the washout-induced myocardial damage is not clear yet, but the H2O2-induced change in prostanoid release did not seem to be responsible for the damage.

Source: Pathophysiology

DOI: 10.1016/s0928-4680(98)00034-0

Authors: Takao Okada, Katsuhiko Asanuma, Tai Nakamura, Rikuo Ochi

Institution: Juntendo University

Language: English

Topic: Cardiac Ischemia and Reperfusion

Subfield: Cardiology and Cardiovascular Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,689