Year: 2010
Type: article
Abstract: We investigated the effect of tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) buffer (pH 7.0) as a bulk solution on the adsorption of DNA by gibbsite, goethite, montmorillonite, kaolinite, synthetic and natural allophanes, two humic acids and two andosols. The natural allophane, gibbsite, kaolinite and an andosol adsorbed significantly more DNA in a 0.1 M Tris-HCl buffer than in a 0.1 M NaCl solution (t-test, P<0.005). In contrast, montmorillonite adsorbed significantly less DNA in the Tris-HCl than NaCl solution (P<0.05). Care should be taken when using Tris-HCl in studies on the adsorption of extracellular DNA molecules by soil particles.
Source: Microbes and Environments
Authors: Kazutoshi Saeki, Takashi Kunito, Masao Sakai
Institutions: Kyushu University, Shinshu University, Kagoshima University
Language: English
Subfield: Water Science and Technology
Field: Environmental Science
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2089655412