Work

Comparative effects in vivo of recombinant murine interleukin 3, natural murine colony-stimulating factor-1, and recombinant murine granulocyte-macrophage colony-stimulating factor on myelopoiesis in mice.

Year: 1987

Type: article

Abstract: Purified murine colony-stimulating factors (CSF) recombinant interleukin 3 (IL-3), natural CSF-1, and recombinant granulo- cyte-macrophage (GM) CSF were assessed in vivo for their ef- fects on BDF, mouse bone marrow and spleen granulocyte-mac- rophage (CFU-GM), erythroid (BFU-E), and multipotential (CFU-GEMM) progenitor cells in untreated mice and in mice pretreated with purified iron-saturated human lactoferrin (LF).The CSF and LF preparations did not contain detectable endo- toxin (< 0.1 ng).Mice pretreated with LF were more sensitive to the effects of CSF.In mice pretreated with LF, 2,000 U IL-3 or 20,000 U CSF-1 significantly enhanced the cycling status and absolute numbers ofall progenitors, whereas 20,000 U GM- CSF significantly increased the cycling status of CFU-GM and CFU-GEMM, but had no effect on cycling of BFU-E or on num- bers of any of the progenitors.The effects of CSF in mice pre- treated with LF were not mimicked by 0.1-100 ng E. co li po- polysaccharide.ministered IL-3, CSF-1, and GM-CSF.Methods Mice.(C57BL/6 X DBA/2)F1 (BDF1) mice, 6-8 wk old, were purchased from Cumberland View Farms (Clinton, TN).Molecules.Murine IL-3 and GM-CSF were expressed from cloned cDNA prepared from RNA extracted from LBRM-33-5A4 cells.The two cDNA clones had nucleotide sequences that were essentially identical to those reported by others for their respective factors (28, 38).The factors were produced in a yeast expression system that used the prepro Effects ofColony-stimulating Factors In Vivo 721

Source: Journal of Clinical Investigation

DOI: 10.1172/jci112877