RAPD analysis for genetic diversity of two populations of Mystus vittatus (Bloch) of Madhya Pradesh, India
Year: 2009
Type: article
Abstract: The random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied to analyze the genetic variation of the 2 populations of Mystus vittatus (Bloch) of Madhya Pradesh, India. 10 random 10-mer primers were primarily scored in 3 individuals from each of the 2 locations. Five primers, which gave polymorphism, were selected for polymerase chain reaction (PCR) and used in the final RAPD analysis of 20 individuals collected from 2 sites, namely Bhadwada reservoir (Bhopal) and Mohinisagar reservoir (Gwalior). These primers produced 388 scorable DNA fragments were found, of which 252 (64.98%) were polymorphic, 182 (46.90%) were monomorphic and 14 (3.61%) were unique. RAPD banding patterns, showed variations between and within the populations, while, the morphological variations were negligible.
Source: AFRICAN JOURNAL OF BIOTECHNOLOGY
DOI: 10.5897/ajb09.604
Authors: R. K. Garg, Nipun Silawat, Pramod Sairkar, Neetu Vijay, Nitin Mehrotra
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2097106940