PaenibacillusBRF-1 has biocontrol ability againstPhialophora gregatadisease and promotes soybean growth
Year: 2008
Type: article
Abstract: Gram-positive bacterium BRF-1 isolated from the rhizosphere of root-rotten diseased soybean plants shows strong antifungal activity on plates. In the present study, the ability of BRF-1 to control brown stem rot disease and to promote plant growth was examined using soybean plants grown in pots. Brown stem rot is a severe soil-borne disease of soybean that is caused by the pathogenic fungus Phialophora gregata. Eighteen-day-old soybean seedlings were grown in a mixture of soil (40 mL) and BRF-1 suspension (10 mL) and were dipped in a suspension of P. gregata (106 c.f.u. mL−1), and the severity of the disease was judged 62 days after the fungal inoculation. Inoculation with BRF-1 at 107 and 108 c.f.u. mL−1 significantly lowered the severity of the disease. Soybean seeds incubated in 100 mL of the BRF-1 suspension (2 × 106 c.f.u. mL−1) were sown in pots filled with sand, and plants were collected 7 days after emergence. The dry weight increase in shoots and roots and mineral absorption were significantly stimulated by the inoculation. These results suggest that BRF-1 might be an effective biocontrol agent to manage brown stem rot disease in soybean. The 16S rDNA sequence of BRF-1 showed high homology with sequences of Paenibacillus polymyxa strains (e.g. 99.7% identity with P. polymyxa strain WY110).
Source: Soil Science & Plant Nutrition
Authors: Keqin Zhou, Masumi Yamagishi, Mitsuru Osaki
Institution: Hokkaido University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2098286356