Work

Estimation of the Cellular Antioxidant Response to Chromium Action Using ESR Method

Year: 2004

Type: article

Abstract: In the present study, the antioxidant capacity of chromium-treated L-41 (human epithelial-like cells) was investigated by the ESR spin-trapping technique. The crude cell extracts of the cells grown in the presence of 2 microM (nontoxic) and 20 microM (toxic) chromium (VI) concentrations were tested in the model Fenton system with and without catalase-inhibitor sodium azide. The presented approach using the ESR technique along with inhibitors lets us discern cell extract defense capacity connected with the enzymatic activity in viable cells and the catabolic activity in dying cells.

Source: The Scientific World JOURNAL

DOI: 10.1100/tsw.2004.136