Work

Development of a sensitive assay for SERCA activity using FRET detection of ADP

Year: 2014

Type: article

Abstract: A SERCA activity assay which detects ADP production via time-resolved FRET is reported, improving sensitivity 50-fold and sample volume 1000-fold.

Source: Analytical Methods

DOI: 10.1039/c3ay41808a

Authors: Meng Jing, Raffaello Verardi, Gianluigi Veglia, Michael T. Bowser

Institution: University of Minnesota

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: Structural Basis Of Dilated Cardiomyopathy, Microfluidic Selection of Aptamers, NMR Structural Analysis of Calcium Regulation in Muscle

OpenAlex ID: W2103720182

FWCI:

Cites: 35

Cited by: 2

Related to: 10