Large-Scale Phenomics Identifies Primary and Fine-Tuning Roles for CRKs in Responses Related to Oxidative Stress
Year: 2015
Type: article
Abstract: Cysteine-rich receptor-like kinases (CRKs) are transmembrane proteins characterized by the presence of two domains of unknown function 26 (DUF26) in their ectodomain. The CRKs form one of the largest groups of receptor-like protein kinases in plants, but their biological functions have so far remained largely uncharacterized. We conducted a large-scale phenotyping approach of a nearly complete crk T-DNA insertion line collection showing that CRKs control important aspects of plant development and stress adaptation in response to biotic and abiotic stimuli in a non-redundant fashion. In particular, the analysis of reactive oxygen species (ROS)-related stress responses, such as regulation of the stomatal aperture, suggests that CRKs participate in ROS/redox signalling and sensing. CRKs play general and fine-tuning roles in the regulation of stomatal closure induced by microbial and abiotic cues. Despite their great number and high similarity, large-scale phenotyping identified specific functions in diverse processes for many CRKs and indicated that CRK2 and CRK5 play predominant roles in growth regulation and stress adaptation, respectively. As a whole, the CRKs contribute to specificity in ROS signalling. Individual CRKs control distinct responses in an antagonistic fashion suggesting future potential for using CRKs in genetic approaches to improve plant performance and stress tolerance.
Source: PLoS Genetics
Authors: Gildas Bourdais, Paweł Burdiak, Adrien Gauthier, Lisette Nitsch, Jarkko Salojärvi, Channabasavangowda Rayapuram, Niina Idänheimo, Kerri Hunter, Sachie Kimura, Ebe Merilo, Aleksia Vaattovaara, Krystyna Oracz, David Kaufholdt, Andres Pallon, Damar Tri Anggoro, Dawid Glów, Jennifer Lowe, Ji Zhou, Omid Mohammadi, Tuomas Puukko, Andreas Albert, Hans Lang, Dieter Ernst, Hannes Kollist, Mikael Brosché, Jörg Durner, Jan Willem Borst, David B. Collinge, Stanisław Karpiński, Michael F. Lyngkjær, Silke Robatzek, Michael Wrzaczek, Jaakko Kangasjärvi
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,043
Funders: Helsingin Yliopisto, Gatsby Charitable Foundation, Deutsche Forschungsgemeinschaft, Fundacja na rzecz Nauki Polskiej, Eesti Teadusagentuur, Academy of Finland, Nederlandse Organisatie voor Wetenschappelijk Onderzoek, Szkola Glówna Gospodarstwa Wiejskiego w Warszawie, Directorate for Biological Sciences, Biotechnology and Biological Sciences Research Council, European Regional Development Fund, Helmholtz Zentrum München, FP7 Research Potential of Convergence Regions
OpenAlex ID: W2103995152