Development and Molecular Characterization of Cystic Hygroma Cell Line to be Used as an in Vitro Model System to Study the Progression of Hygroma in Young Children
Year: 2013
Type: article
Abstract: Cystic hygroma is a non-malignant tumour of the lymphatic system which occurs at sites when the lymphatic system fails to interact with venous system.This disorder is frequent in infancy but rare in adults.The etiology of this disorder is not known and no specific therapy exists.Therefore, there is an urgent need to develop an in vitro model system from cystic hygroma tumour cells to understand molecular mechanism of this disorder specifically in young children.In the present study, we have isolated morphologically distinct cells which have tumorigenic potential from cystic hygroma tumour.The tissue and the cells were characterized using specific molecular markers.Our data showed that these cells expressed Nanog, Keratine18, CD73, CD34, CD44, EGFR, TNF-α and Vimentin both in vivo and in vitro cells.However, Oct4, C-kit, SSEA-4, Ki-67 and VEGF-A are expressed only in vitro cells.IL-6 is highly expressed in in vitro cells indicating their pro-inflammatory response in cystic hygroma cells.This study reports for the first time the specific molecular markers which are expressed in the cells and tissue of cystic hygroma tumour which may be responsible for the tumour development.Immunofluorescence microscopy confirms the expression of two proteins i.e.EGFR and Ki-67 which are indicative of benign status of cystic hygroma.Therefore, human cystic hygroma cell line represents a suitable in vitro model system to study the mechanism of cystic hygroma tumour development and to identify and design a specific molecular target for cystic hygroma therapies in young children in near future.
DOI: 10.5171/2013.879081
Institution: Jaslok Hospital
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W2107367449