Work

Mutagenesis Analysis of a Conserved Region Involved in Acetyl Coenzyme A Binding in the Aminoglycoside 6′- N -Acetyltransferase Type Ib Encoded by Plasmid pJHCMW1

Year: 2005

Type: article

Abstract: Alanine scanning of motif A in the pJHCMW1-encoded aminoglycoside 6'-N-acetyltransferase type Ib identified amino acids important for the ability of the enzyme to confer wild-type levels of resistance to kanamycin and amikacin. The replacement of two amino acids, D117 or L120, with alanine residues resulted in complete loss of the resistance phenotype.

Source: Antimicrobial Agents and Chemotherapy

DOI: 10.1128/aac.49.7.2979-2982.2005

Authors: Atousa Pourreza, Mavee Witherspoon, Jessica Fox, Jason Newmark, Duyen Bui, Marcelo E. Tolmasky

Institution: California State University, Fullerton

Language: English

Topic: Antibiotic Resistance in Bacteria

Subfield: Microbiology (medical)

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases

Awards: Minority Student Development at CSU Fullerton, G3803844883, G5618224283, Dealing with antibiotic resistance: antisense technology