Work

Transient expression of homologous hairpin RNA interferes with PVY transmission by aphids

Year: 2008

Type: article

Abstract: Hairpin RNAs have been used to confer resistance to viruses in plants through RNA silencing. However, it has not been demonstrated that RNA silencing was effective against inoculation by aphids of non-persistently transmitted viruses, the major route of plant virus spread in nature. As a proof-of-principle strategy, we made use of Agrobacterium tumefaciens to transiently express a hairpin RNA homologous to Potato virus Y (PVY) in plant tissues. A complete and specific interference with aphid transmission of PVY was achieved by inducers of RNA silencing, as demonstrated by specific siRNAs accumulation in agroinfiltrated tissues. To our knowledge, this is the first report of successful interference with non-persistent transmission of a plant virus using RNA interference.

Source: Virology Journal

DOI: 10.1186/1743-422x-5-42

Authors: Marisol Vargas, Belén Martínez-García, José Ramón Díaz-Ruíz, Francisco Tenllado

Institutions: Consejo Superior de Investigaciones Científicas, Centro de Investigaciones Biológicas Margarita Salas

Language: English

Topic: Plant Virus Research Studies

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $3,290