Sources of energy and energy coupling reactions of the active transport systems in Acinetobacter calcoaceticus.
Year: 1986
Type: article
Abstract: using a H+-ATPaseactivity-deficient mutant in the presence of variable metabolic inhibitors.6'7*Recently, we selected the tributyltin-resistant mutant of A. calcoa-ceticus8) which is deficient in H+-ATPase activity.This paper describes the properties of the active transport systems of A. calcoaceticus.A. calcoaceticus K-104 was obtained from the Institute of Microbiology, Kyoto. A. calcoaceticus K-104A, obtained through natural mutation ofK-104, was deficient in H+-ATPase activity.8)Tributyltin (4 x 10~3% (v/v)) was used for the isolation of the mutant.9)These organisms were grown in Stanier's minimum medium10) containing permiable intermediates of the tricarboxylic acid cycle.The rates ofO2 uptake by intact cells were measured in a vessel equipped with an oxygen electrode probe.The
Authors: Kouichiro KITAGAWA, Akiko TATEISHI, Fusako NAKANO, Takuya MATSUMOTO, Toshiro MOROHOSHI, Teruo TAKING, Touru Usui
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2115071376