Studies of temperature-dependent electronic transduction on DNA hairpin loop sensor
Year: 2003
Type: article
Abstract: A self-assembly monolayer (SAM) of hairpin DNA can be formed on a gold substrate in order to make special biosensors. Labeling the hairpin loop probes with electroactive compositions rather than a fluorophore illustrates interesting profiles of redox current versus temperature. For a biosensor interacting with perfectly complementary targets, the profile shows a characteristic plateau, which disappears when the targets have a single base variation. The plateau is split into multiple steps by tuning the hybridization temperature. We propose that the phenomena are due to hairpin loop compartmentalization. The novel characteristics lead to a thermal gradient detection method that permits perfect discrimination of a target sequence from single nucleotide mismatches.
Source: Nucleic Acids Research
DOI: 10.1093/nar/gng108
Author: Y. Mao
Institution: Peking University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funder: Peking University
OpenAlex ID: W2116232136