Work

Rapid and Simple Cryopreservation of Anaerobic Ammonium-Oxidizing Bacteria

Year: 2012

Type: article

Abstract: A quick and simple protocol for long-term cryopreservation of anaerobic ammonium-oxidizing bacteria (anammox bacteria) was developed. After 29 weeks of preservation at -80°C, activity recovery for all tested cultures under at least one of the applied sets of preservation conditions was observed. Growth recovery was also demonstrated for a single-cell culture of "Candidatus Kuenenia stuttgartiensis."

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.07501-11

Authors: Kim Heylen, Katharina Ettwig, Ziye Hu, Mike Jetten, Boran Kartal

Institutions: Radboud University Nijmegen, Ghent University, Delft University of Technology

Language: English

Topic: Wastewater Treatment and Nitrogen Removal

Subfield: Pollution

Field: Environmental Science

Domain: Physical Sciences

Open Access status: bronze

Funder: Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Awards: Preservation of difficult to grow aerobic and anaerobic microbes. Bezoeker: Dr. K. Heylen., A major source of nitrogen for our atmosphere: molecular mechanism of anaerobic ammonium oxidation