Work

Pharmacological inhibition of Aurora-A but not Aurora-B impairs interphase microtubule dynamics

Year: 2009

Type: article

Abstract: Aurora kinases are key cell cycle regulators and represent attractive new targets in cancer therapy. In this work we investigated the effect of specific inhibition of Aurora-A and Aurora-B on interphase microtubule dynamics using the GSK6000063A and AZD1152 HQPA compounds respectively. We show that Aurora-A inhibition results in microtubule network disorganization and bundling. Using video microscopy and laser-based photo ablation we demonstrate that Aurora-A inhibition decreases microtubule shrinkage, growth rate, frequency rescue and nucleation. These results open new perspectives on the role of Aurora-A in interphase and might be worth considering in a pharmacological perspective.

Source: Cell Cycle

DOI: 10.4161/cc.8.11.8617

Authors: Corinne Lorenzo, Qiaoyin Liao, Mary Ann Hardwicke, Bernard Ducommun

Institutions: Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération, Université de Toulouse, GlaxoSmithKline (United States), Hôpital Purpan

Language: English

Topic: Microtubule and mitosis dynamics

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Ligue Contre le Cancer, Conseil Régional Midi-Pyrénées