Work

Colony Multiplex PCR Assay for Identification and Differentiation of Campylobacter jejuni , C. coli , C. lari , C. upsaliensis , and C. fetus subsp . fetus

Year: 2002

Type: article

Abstract: A multiplex PCR assay was used to simultaneously detect genes from the five major clinically relevant Campylobacter spp. Those genes selected were hipO and 23S rRNA from Campylobacter jejuni; glyA from each of C. coli, C. lari, and C. upsaliensis; and sapB2 from C. fetus subsp. fetus. The assay was evaluated with 137 clinical and environmental isolates and was found to be rapid and easy to perform and had a high sensitivity and specificity for characterizing isolates, even in mixed cultures.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.40.12.4744-4747.2002

Authors: Gehua Wang, Clifford G. Clark, Tracy M. Taylor, Chad Pucknell, Connie Barton, Lawrence Price, David L. Woodward, Frank G. Rodgers

Language: English

Topic: Salmonella and Campylobacter epidemiology

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2124226882

FWCI: 7.132

Cites: 24

Cited by: 516

Related to: 10