Work

Determining the Viability of Zebra Mussel (dreissena Polymorpha) Spermatozoa and Changes in the Integrity of Its Plasma Membrane Using the Fluorescence Method

Year: 2004

Type: article

Abstract: The viability of zebra mussel (Dreissena polymorpha) sperm and changes in the integrity of its plasma membrane were examined using combined nucleic fluorescent stains SYBR-14 and propidium iodide. Approximately 97 ± 3% of the sperm in freshly ejaculated zebra mussel semen was stained with SYBR-14 (potentially viable). A statistically significant decrease in plasma membrane integrity was observed after 48 hours (8°C) when 42 ± 11% of the sperm stained as viable. Semen stored at room temperature (20°C) lost its plasma membrane integrity after 4 hours (0% viable). Motility declined from 60 ± 4% (0 time) to 30 ± 3% after 24 hours. The percentage of sperm stained with SYBR-14 was not correlated with sperm motility or the fertility test.CORRESPONDING AUTHOR: Joanna Białkowska, Uniwersytet Warmińsko-Mazurski, Zakład Genetyki Ewolucyjnej, ul. Oczapowskiego 5, 10-957 Olsztyn, tel./fax: +48 89 5233754; e-mail: [email protected]

Source: Fisheries & Aquatic Life

Authors: Joanna Białkowska, W. Demianowicz, J. Głogowski

Institutions: University of Warmia and Mazury in Olsztyn, InLife Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Polish Academy of Sciences

Language: English

Topic: Reproductive biology and impacts on aquatic species

Subfield: Physiology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Life below water

Open Access status: diamond

OpenAlex ID: W2135242571

FWCI:

Cites: 10

Cited by: 2

Related to: 10