Year: 1995
Type: article
Abstract: A thermostable extracellular glucoamylase from the thermophilic fungus Humicola grisea was purified to homogeneity. Its molecular mass and isoelectric point were 74 kDa and 8.4, respectively. The enzyme contained 5% carbohydrate, showed maximal activities at pH 6.0 and 60(deg)C, and was stable at 55(deg)C and pH 6.0 for 2 h. The K(infm) of soluble starch hydrolysis at 50(deg)C and pH 6.0 was 0.14 mg/ml. The purified enzyme was remarkably insensitive to glucose.
Authors: L Campos, C R Felix
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2137053293