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Influence of pr-M Cleavage on the Heterogeneity of Extracellular Dengue Virus Particles

Year: 2010
Type: article
Abstract: During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage.
DOI: 10.1128/jvi.00696-10
Authors: Jiraphan Junjhon, Thomas J. Edwards, Utaiwan Utaipat, Valorie D. Bowman, Heather A. Holdaway, Wei Zhang, Poonsook Keelapang, Chunya Puttikhunt, Rushika Perera, Paul R. Chipman, Watchara Kasinrerk, Prida Malasit, Richard J. Kuhn, Nopporn Sittisombut
Institutions: Chiang Mai University, Purdue University West Lafayette, National Science and Technology Development Agency, National Center for Genetic Engineering and Biotechnology, Siriraj Hospital, Mahidol University
Language: English
Field: Medicine
Open Access status: green
OpenAlex ID: W2137755244
FWCI: 13.33
Cites: 38
Cited by: 188
Related to: 10