Year: 2014
Type: article
Abstract: The ability to conditionally direct antibodies is a potentially powerful application for Synthetic Biology in Medicine. Here we show that control of antibody binding through site-specific, chemical phosphorylation of a recognition domain creates a ‘gated’ antibody (Ab). This displays a crude Boolean logic where binding is induced in an enzyme-AND-antigen dependent manner. This ‘AND-Ab’ is therefore active only in the presence of two biomarker inputs: the simultaneous expression of a (cell surface) antigen and secreted enzyme to generate function in vitro, on cells and in mammalian tissue. Such gated Abs, either alone or in combination, could allow the application of logic strategies to enhance precision in biological interrogation, modulation and in therapy. The ability to control antibody binding could have important medical implications. Here, the authors present a method to engineer phosphatase-controllable antibodies that bind to a specific recognition site in the presence of two biomarker inputs.
Source: Nature Communications
DOI: 10.1038/ncomms5388
Institutions: University of Oxford, UCB Pharma (United Kingdom)
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $6,990
Awards: G2251270888, G295399147, G3038452519, Engineering stable calibration standards for biomedical research, Dissecting the mechanism by which glycosyltransferases calalyse mannosyl transfer, G7739863634, The CHELL : A Bottom-Up approach to in vitro and in silico Minimal Life-like Constructs, Creating a Synthetic Platform for Understanding and Exploiting Glycoconjugates, G8603049439
OpenAlex ID: W2138148696