Work

Unidirectional Cre-mediated genetic inversion in mice using the mutant loxP pair lox66/lox71

Year: 2003

Type: article

Abstract: The Cre/loxP recombination system is a commonly used tool to alter the mouse genome in a conditional manner by deletion or inversion of loxP-flanked DNA segments. While Cre-mediated deletion is essentially unidirectional, inversion is reversible and therefore does not allow the stable alteration of gene function in cells that constitutively express Cre. Site-directed mutagenesis yielded a pair of asymmetric loxP sites (lox66 and lox71) that display a favorable forward reaction equilibrium. Here, we demonstrate that lox66/lox71 mediates efficient and predominantly unidirectional inversion of a switch substrate targeted to the mouse genome in combination with either inducible or cell type-specific cre-transgenes in vivo.

Source: Nucleic Acids Research

DOI: 10.1093/nar/gng140

Author: P. Oberdoerffer

Institution: Harvard University

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Cancer Institute, National Institute of Allergy and Infectious Diseases

Awards: G2052491892, PROGRAM PROJECT GRANT: Molecular Targets of Germinal Center B-Cell Lymphomas, G7319916932, G9000055271, G9000071208