Work

Immobilization of Actively Thromboresistant Assemblies on Sterile Blood‐Contacting Surfaces

Year: 2013

Type: article

Abstract: Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.

Source: Advanced Healthcare Materials

DOI: 10.1002/adhm.201300110

Authors: Zheng Qu, Venkat Krishnamurthy, Carolyn A. Haller, Brent M. Dorr, Ulla M. Marzec, Sawan Hurst, Monica T. Hinds, Stephen R. Hanson, David R. Liu, Elliot L. Chaikof

Institutions: Georgia Institute of Technology, Beth Israel Deaconess Medical Center, Harvard University, The Wallace H. Coulter Department of Biomedical Engineering, Wyss Institute for Biologically Inspired Engineering, Howard Hughes Medical Institute, Oregon National Primate Research Center, Oregon Health & Science University

Language: English

Topic: Biochemical and Structural Characterization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute

Awards: Site-Specific Therapies To Prevent Intimal Hyperplasia, In Situ Regeneration of Bioactive Surfaces: Rechargeable Anti-thrombogenic Films, A Bioinspired Small Diameter Vascular Conduit, Support for National Primate Research Center

OpenAlex ID: W2139787099

FWCI: 1.714

Cites: 63

Cited by: 30

Related to: 10