Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood
Year: 2011
Type: article
Study design: Observational Study
Abstract: Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease. In our studies, we have access to DNA samples extracted from human saliva and blood. In this report, we tested a real-time PCR assay developed to detect copies of genomic DNA from Streptococcus mutans in 1,424 DNA samples from humans. Our results suggest that we can determine the presence of genomic DNA copies of Streptococcus mutans in both DNA samples from caries-free and caries-affected individuals. However, we were not able to detect the presence of genomic DNA copies of Streptococcus mutans in any DNA samples extracted from peripheral blood, which suggests the assay may not be sensitive enough for this goal. Values of the threshold cycle of the real-time PCR reaction correlate with higher levels of caries experience in children, but this correlation could not be detected for adults.
Source: ISRN Dentistry
DOI: 10.5402/2011/543561
Authors: Alexandre R. Vieira, Kathleen B. Deeley, Nicholas F. Callahan, Jacqueline B. Noel, Ida Anjomshoaa, Wendy M. Carricato, Louise P. Schulhof, Rebecca S. DeSensi, Pooja Gandhi, Judith M. Resick, Carla A. Brandon, Christopher Rozhon, Asli Patir, Mine Yildirim, Fernando A. Poletta, Juan C. Mereb, Ariadne Letra, Renato Menezes, Steven Wendell, Jorge S. Lopez-Camelo, Eduardo E. Castilla, Iêda M. Orioli, Figen Seymen, Robert J. Weyant, Richard Crout, Daniel W. McNeil, Adriana Modesto, Mary L. Marazita
Institutions: University of Pittsburgh, Istanbul Medipol University, Istanbul University, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Centro de Educación Médica e Investigaciones Clínicas Norberto Quirno, Instituto Multidisciplinario de Biología Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Universidade Federal do Rio de Janeiro, West Virginia University
Language: English
Subfield: Periodontics
Field: Dentistry
Domain: Health Sciences
Open Access status: diamond
Funders: University of Pittsburgh, Consejo Nacional de Investigaciones Científicas y Técnicas, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Instituto Nacional de Genética Médica Populacional, National Institutes of Health, School of Dental Medicine, University of Pittsburgh, Agencia Nacional de Promoción Científica y Tecnológica, National Institute of Dental and Craniofacial Research, National Center for Research Resources, National Center for Advancing Translational Sciences
Awards: University of Pittsburgh Clinical and Translational Science Institute, G2253849403, Craniofacial Anomalies Research Center, G2724527233, Extending the Phenotype of Nonsyndromic Orofacial Clefting, Factors Contributing to Oral Health Disparities in Appalachia, G5417900476, G6064507860, G7378882120, University of Pittsburgh Clinical and Translational Science Institute, Genetics of Caries
OpenAlex ID: W2140505190