In vitro rapid and mass multiplication of highly valuable medicinal plant Bacopa monnieri (L.) Wettst.
Year: 2010
Type: article
Abstract: A protocol has been developed for micropropagation of Bacopa monnieri (L) Wettst, a medicinal plant of high commercial potential with legendary reputation as a memory vitalizer. Nodal segments containing axillary buds were surface sterilized with 0.1% solution of mercuric chloride for 5 min and were inoculated aseptically on culture medium, axillary bud break was achieved in 100% of cultures in semisolid MS medium supplemented with 0.2 mg/l BAP. These proliferated in vitro axillary shoots were excised and cut into groups of shoot clusters and subcultured on MS medium supplemented with 0.2 mg/l BAP for shoot multiplication. 100% in vitro rooting was obtained when shoot clusters were cultured on MS medium supplemented with 0.15 mg/l IBA. The rooted plantlets were hardened, acclimatized and successfully established in field. Key words : Bacopa monnieri , nodal segments, micropropagation, plant growth regulators.
Source: AFRICAN JOURNAL OF BIOTECHNOLOGY
DOI: 10.5897/ajb09.878
Authors: Sudhir Kumar Sharma, Barkha Kamal, Neelima Rathi, Sudhir Chauhan, Vikas Jadon, Neha Vats, Ashok Gehlot, Sarita Arya
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2149457259