Work

Transcriptomic profiles of Drosophila melanogaster third instar larval midgut and responses to oxidative stress

Year: 2008

Type: article

Abstract: Oligoarray analysis was used to determine the number and nature of genes expressed in third instar Drosophila melanogaster larval midguts. The majority of transcripts were associated with protein synthesis and metabolism. Serine proteases were the main proteolytic enzymes detected. Some 40% of the cytochrome P450 genes and 74% of the glutathione S transferases (GSTs) in the genome of D. melanogaster were observed to be expressed in the midgut by oligoarray analysis. We also identified potential transcription factor binding motifs (TFBMs) of P450s, GSTs and carboxylesterases. Many of the midgut-expressed GST genes contained candidate TFBMs homologous to TFBMs in mammals that have been associated with responses to oxidative stress. We also investigated the response of GSTs in the midgut to dietary H2O2, which showed a dosage-based differential response.

Source: Insect Molecular Biology

DOI: 10.1111/j.1365-2583.2008.00808.x

Authors: H.‐M. Li, G. Buczkowski, O. Mittapalli, J. Xie, J. Wu, R. Westerman, B. J. Schemerhorn, L. L. Murdock, B. R. Pittendrigh

Institutions: Purdue University West Lafayette, Max Planck Institute for Chemical Ecology, Agricultural Research Service, United States Department of Agriculture

Language: English

Topic: Glutathione Transferases and Polymorphisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: U.S. Department of Agriculture, Purdue University

OpenAlex ID: W2151443015

FWCI: 2.73

Cites: 77

Cited by: 103

Related to: 10