Work

Focusing Transgene Expression in Drosophila by Coupling Gal4 With a Novel Split-LexA Expression System

Year: 2011

Type: article

Abstract: Here we report the development of a ternary version of the LexA::VP16/LexAop system in which the DNA-binding and trans-activating moieties are independently targeted using distinct promoters to achieve highly restricted, intersectional expression patterns. This Split LexA system can be concatenated with the Gal4/upstream activating sequence system to refine the expression patterns of existing Gal4 lines with minimal genetic manipulations.

Source: Genetics

DOI: 10.1534/genetics.110.126193

Authors: Chun-Yuan Ting, Stephanie Gu, Sudha Guttikonda, Tzu-Yang Lin, Benjamin H White, Chi-Hon Lee

Institutions: National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institute of Mental Health

Language: English

Topic: Neurobiology and Insect Physiology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Mental Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Awards: G4965740557, Identifying Neural Substrates of Behavior in Drosophila Melanogaster, Cellular And Molecular Mechanism Of R7 Target Selection