Efficacy of Cryptolepis sanguinolenta root extract on slow-growing rifampicin resistant Mycobacterium tuberculosis
Year: 2012
Type: article
Abstract: We report here, the results from total crude methanol extract as well as serial ether, chloroform, and \nmethanol extracts of the root of Cryptolepis sanguinolenta (Lindl) Schltr that were screened against \nthree strains of Mycobacterium tuberculosis. The strains used included the pan sensitive H37Rv, the \nrifampicin-resistant TMC-331 and a wild strain of Mycobacetrium avium (MA) isolated from a Ugandan \npatient. The disc diffusion method was used for susceptibility tests on solid Middle brook 7H10 while \nthe minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were \ndetermined by the microtitre plate method using Middle brook 7H9 broth. We report that the total crude \nmethanol extract showed the highest activity against H37Rv and TM-331 with complete clearance of \nquadrants at 50 mg/ml and zones of inhibition of 10.0 to 11 mm at 25 mg/ml concentration although it \nwas not effective against M. avium. The corresponding MIC values were 1.17 mg/ml for H37Rv and 1.56 \nmg/ml for TMC-331. The values for isoniazid were 0.25 and 9.38 µg/ml for H37Rv and TMC-331, \nrespectively, while for rifampicin the MIC value was 0.25 µg/ml for H37Rv but it was not active on TMC- \n331. Acute toxicity test gave an LD50 of 758.5 mg/kg body weight while the phytochemical analysis \nshowed the presence of alkaloids, tannins and flavonoids.
DOI: 10.5897/jmpr10.856
Author: Kirimuhuzya Claude
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W2162000223