Work

Endothelial Differentiation of Embryonic Stem Cells

Year: 2008

Type: article

Abstract: Vascular progenitor cells derived from stem cells could potentially lead to a variety of clinically relevant applications, including cell-based therapies and tissue engineering. Here, we describe methods for isolating purified proliferating populations of vascular endothelial cells from mouse embryonic stem cells (mESC) using Flk-1 positive sorted cells, VEGF supplementation, and a rigorous manual selection technique required for endothelial cell purification and expansion. Using this in vitro derivation procedure, it is possible to obtain millions of cells at various stages of differentiation, with the potential for up to 25 population doublings.

Source: Current Protocols in Stem Cell Biology

DOI: 10.1002/9780470151808.sc01f05s6

Authors: Alicia A. Blancas, Nicholas E. Lauer, Kara E. McCloskey

Institution: University of California, Merced

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Heart, Lung, and Blood Institute