Overdrive is a T-region transfer enhancer which stimulates T-strand production inAgrobacterium tumefaciens
Year: 1987
Type: article
Abstract: Introduction of a left or right synthetic border repeat together with the overdrive sequence in an octopine Ti-plasmid deletion mutant, lacking the right border, resulted in the complete restoration of the oncogenicity of the mutant strain. However introduction of a border repeat without the overdrive, only restored oncogenicity partially. The overdrive sequence turned out to be able to stimulate the synthetic border mediated T-region transfer, independent of its orientation and position relative to the border repeat. Furthermore the distance between border repeat and overdrive could be enlarged, without a loss of overdrive activity. Here we enlarged the distance between the two sequences up to 6714bp. These results were confirmed by estimating the amount of single stranded T-DNA molecules from induced agrobacteria, containing the various border constructs.
Source: Nucleic Acids Research
Institution: Leiden University
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
Funder: Genentech
OpenAlex ID: W2164167151