Work

Chemistry-based RNA technologies: demonstration of usefulness of libraries of ribozymes and short hairpin RNAs (shRNAs)

Year: 2005

Type: conference-paper

Abstract: Mechanism of action of hammerhead ribozymes has been investigated and their intracellular activities have been improved. Based on the improved ribozymes and more recently discovered natural RNAi, we have created libraries of both ribozymes and short hairpin RNAs (shRNAs). The introduction of a library of active ribozymes or shRNAs into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function.

Source: Nucleic Acids Symposium Series

DOI: 10.1093/nass/49.1.91

Authors: Makoto Miyagishi, Sahohime Matsumoto, Hideo Akashi, Hiroaki Kawasaki, Taro Fukao, Yoko Fukuda, Masayuki Sano, Yoshio Kato, Yasuomi Takagi, Yoshiyuki Tanaka, Masaki Warashina, Tomoko Kuwabara, Shinya Y. Sawata, Yutaka Ikeda, Shun-ichi Kawahara, Kaul C. Sunil, Renu Wadhwa, Kazunari Taira

Institution: The University of Tokyo

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2167954883

FWCI: 3.095

Cites:

Cited by: 5

Related to: 10