Detection of Circulating Tumor DNA in Early- and Late-Stage Human Malignancies
Year: 2014
Type: article
Study design: Observational Study
Abstract: The development of noninvasive methods to detect and monitor tumors continues to be a major challenge in oncology. We used digital polymerase chain reaction-based technologies to evaluate the ability of circulating tumor DNA (ctDNA) to detect tumors in 640 patients with various cancer types. We found that ctDNA was detectable in >75% of patients with advanced pancreatic, ovarian, colorectal, bladder, gastroesophageal, breast, melanoma, hepatocellular, and head and neck cancers, but in less than 50% of primary brain, renal, prostate, or thyroid cancers. In patients with localized tumors, ctDNA was detected in 73, 57, 48, and 50% of patients with colorectal cancer, gastroesophageal cancer, pancreatic cancer, and breast adenocarcinoma, respectively. ctDNA was often present in patients without detectable circulating tumor cells, suggesting that these two biomarkers are distinct entities. In a separate panel of 206 patients with metastatic colorectal cancers, we showed that the sensitivity of ctDNA for detection of clinically relevant KRAS gene mutations was 87.2% and its specificity was 99.2%. Finally, we assessed whether ctDNA could provide clues into the mechanisms underlying resistance to epidermal growth factor receptor blockade in 24 patients who objectively responded to therapy but subsequently relapsed. Twenty-three (96%) of these patients developed one or more mutations in genes involved in the mitogen-activated protein kinase pathway. Together, these data suggest that ctDNA is a broadly applicable, sensitive, and specific biomarker that can be used for a variety of clinical and research purposes in patients with multiple different types of cancer.
Source: Science Translational Medicine
Authors: Chetan Bettegowda, Mark Sausen, Rebecca J. Leary, Isaac Kinde, Yuxuan Wang, Nishant Agrawal, Bjarne R. Bartlett, Hao Wang, Brandon Luber, Rhoda M. Alani, Emmanuel S. Antonarakis, Nilofer S. Azad, Alberto Bardelli, Henry Brem, John L. Cameron, Clarence C. Lee, Leslie A. Fecher, Gary L. Gallia, Peter Gibbs, Dung Le, Robert L. Giuntoli, Michael Goggins, Michael D. Hogarty, Matthias Holdhoff, Seung-Mo Hong, Yuchen Jiao, Hartmut H. Juhl, Jenny J. Kim, Giulia Siravegna, Daniel A. Laheru, Calogero Lauricella, Michael Lim, Evan J. Lipson, Suely Kazue Nagahashi Marie, George J. Netto, Kelly S. Oliner, Alessandro Olivi, Louise Olsson, Gregory J. Riggins, Andrea Sartore-Bianchi, Kerstin Schmidt, le-Ming Shih, Sueli Mieko Oba-Shinjo, Salvatore Siena, Dan Theodorescu, Jeanne Tie, Timothy T. Harkins, Silvio Veronese, Tian-Li Wang, Jon D. Weingart, Christopher L. Wolfgang, Laura D. Wood, Dongmei Xing, Ralph H. Hruban, Jian Wu, Peter J. Allen, C. Max Schmidt, Michael A. Choti, Victor E. Velculescu, Kenneth W. Kinzler, Bert Vogelstein, Nickolas Papadopoulos, Luis A. Diaz
Institutions: Howard Hughes Medical Institute, Johns Hopkins University, Johns Hopkins Medicine, Johns Hopkins Hospital, Sidney Kimmel Comprehensive Cancer Center, Boston University, IFOM, Candiolo Cancer Institute, University of Turin, Indiana University Health, Indiana University Hospital, The Royal Melbourne Hospital, Ludwig Cancer Research, Western Hospital, Children's Hospital of Philadelphia, University of Pennsylvania, Asan Medical Center, University of Ulsan, Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda, Universidade de São Paulo, Amgen (United States), Karolinska Institutet, University of Colorado Cancer Center, Memorial Sloan Kettering Cancer Center, Indiana University Indianapolis
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
Awards: G1165598027, G1176235484, G1207848105, G1324540468, G1553386191, G1617166219, G1654613813, G1869329471, G2899213437, Signaling and Progression in Prostate Cancer, G3089612437, G3169672230, G3479938378, Phase I study of Clostridium novyi-NT spores in solid tumor cancer patients, G3900205185, Cyclophosphamide modified GM-CSF pancreatic tumor vaccine + listeria-mesothelin, Characterizing Notch3 Signaling in Ovarian Cancer, G5940631918, G6553408154, G7049171053, G7906264279, Cellular and Molecular Medicine, Genes from the FAP Locus, G8897054222, Molecular Targets for Cancer Detection and Treatment, G970037175
OpenAlex ID: W2171237493