Phenotypic and genomic comparisons of highly vancomycin-resistant Staphylococcus aureus strains developed from multiple clinical MRSA strains by in vitro mutagenesis
Year: 2015
Type: article
Abstract: The development of vancomycin (VCM) resistance in Staphylococcus aureus threatens global health. Studies of the VCM-resistance mechanism and alternative therapeutic strategies are urgently needed. We mutagenized S. aureus laboratory strains and methicillin-resistant S. aureus (MRSA) with ethyl methanesulfonate, and isolated mutants that exhibited high resistance to VCM (minimum inhibitory concentration = 32 μg/ml). These VCM-resistant strains were sensitive to linezolid and rifampicin, and partly to arbekacin and daptomycin. Beta-lactams had synergistic effects with VCM against these mutants. VCM-resistant strains exhibited a 2-fold increase in the cell wall thickness. Several genes were commonly mutated among the highly VCM-resistant mutants. These findings suggest that MRSA has a potential to develop high VCM resistance with cell wall thickening by the accumulation of mutations.
Source: Scientific Reports
DOI: 10.1038/srep17092
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,690
Awards: Comprehensive understanding of bacterial adaptation mechanism in diabetic host, Development of lead commands for new anti-infective agents, Platform for the identification of novel therapeutically effective antimicrobial agents, Establishment of a screening system for therapeutically effective antibiotics, 香川県志度寺義「志度寺縁起の研究」, Research on the relationship between psm-mec mutations and MRSA virulence, G8947531122
OpenAlex ID: W2180304118