Work

Transgenic Drosophila melanogaster Carrying RNAi Against gce Gene Proved the Involvement of gce in Juvenile Hormone Action

Year: 2012

Type: article

Abstract: Juvenilehormone (JH) is important for multiple aspects of insect development andphysiology. Although roles of JH have received considerable studies, JHsignaling pathway at the molecular level is still not well understood. Methoprene-tolerant(Met) in Drosophila melanogaster fulfills many of therequirements as a hormone receptor gene. A paralogous gene, germ-cellexpressed (gce), possesses homology to Met and is a candidateas a Met partner in mediating JH action. To probe roles of this gene inJH action, we carried out in vivo gce underexpression studies using RNAitechnique. Precocious expression of broad (br) gene was found tooccur when gce was knocked down in the young larvae. We also demonstratedthat RNA interference-driven knockdown of gce expression in transgenicflies results in appearance of resistance to pyriproxyfen (IGRs). These resultsshow that gce is a vital gene and appears to promote JH action in larvalstages.

Source: Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology/Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

DOI: 10.21608/eajbsc.2012.16123

Authors: Reda Bakr, Jehane Hafez, Thorya Alnagar, Dalia Salem, Ola Zyaan

Institution: Ain Shams University

Language: English

Topic: Neurobiology and Insect Physiology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $100

OpenAlex ID: W2188186231