Work

Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin

Year: 2015

Type: article

Abstract: Advancing the practical utility of nonlinear optical microscopy requires continued improvement in imaging depth and contrast. We evaluated second-harmonic generation (SHG) and third-harmonic generation images from ex vivo human skin and showed that a sub-40 fs, 1060-nm Yb-fiber laser can enhance SHG penetration depth by up to 80% compared to a >100 fs, 800 nm Ti:sapphire source. These results demonstrate the potential of fiber-based laser systems to address a key performance limitation related to nonlinear optical microscopy (NLOM) technology while providing a low-barrier-to-access alternative to Ti:sapphire sources that could help accelerate the movement of NLOM into clinical practice.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.jbo.20.12.120501

Authors: Mihaela Balu, Ilyas Saytashev, Jue Hou, Marcos Dantus, Bruce J. Tromberg

Institutions: University of California, Irvine, Beckman Laser Institute and Medical Clinic, Michigan State University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $1,675