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Blastocystis ST-1 and ST-3 Mixed Infection Causes Increased Inflammatory Responses and NF-kappaB Protein Expression

Year: 2015

Type: article

Abstract: Background: Blastocystis sp is one of the most common intestinal protozoa found in humans, but its pathogenesis is still unclear. One of the influencing factors is its genetic subtype. The subtypes that most commonly infect humans are subtypes 1-4. Blastocystis subtypes that are commonly found in Indonesia, especially in Padang City, are Blastocystis ST 1 and ST3.Methods: This study was experimental with a post-test-only control group design. A total of 18 rats were randomly divided into 3 sample groups: control group, treatment group with Blastocystis ST 1&3 dose 104 and dose 105. The inflammatory response was assessed by histopathological and immunohistochemical examination of NF-kappaB protein. One-way ANOVA and Kruskal Wallis tests were conducted to determine the significance of differences in the expression of each protein and gene in the three groups of animals.Result: Blastocystis ST 1 and ST 3 mixed infection in mice caused mild-moderate inflammation in histopathological examination of intestinal tissue and increased NF-kappaB protein expression, especially in the dose 105 treatment group (14.45±5.51) compared to the dose 104 treatment group (8.24±2.72) and the control group (7.41±1.21). This difference was statistically significant (P<0.05).Conclusion: This study concluded that Blastocystis ST 1&3 mixed infection can increase the inflammatory response in mice's intestinal tissue. This effect was particularly evident at higher doses 105.Keywords: Blastocystis, Histopathology, Inflammatory, NF-kappaB Protein

Source: Advancements in Life Sciences

DOI: 10.62940/als.v13i2.3455