A radiosensitivity MiRNA signature validated by the TCGA database for head and neck squamous cell carcinomas
Year: 2015
Type: article
Abstract: // Ning Liu 1, 2, * , Rebecca J. Boohaker 1, * , Chunling Jiang 3, 4, * , James R. Boohaker 5 , Bo Xu 1, 6 1 Department of Oncology, Southern Research Institute, Birmingham, AL 35205, USA 2 Department of Gastric Cancer Surgery, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, China 3 Nanchang University Graduate School, Nanchang 330047, China 4 Department of Radiation Oncology, Jiangxi Cancer Hospital, Nanchang 330029, China 5 Department of Economics, American University, Washington, DC 20016, USA 6 Cancer Cell Biology Program, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35205, USA * These authors have contributed equally to this work Correspondence to: Bo Xu, e-mail: [email protected] , e-mail: [email protected] Keywords: miRNA, radiosensitivity, ATM Received: January 29, 2015 Accepted: September 24, 2015 Published: October 06, 2015 ABSTRACT MicroRNA, a class of small non-coding RNAs, play critical roles in the cellular response to DNA damage induced by ionizing irradiation (IR). Growing evidence shows alteration of miRNAs, in response to radiation, controls cellular radiosensitivity in DNA damage response pathways. However, it is less clear about the clinical relevance of miRNA regulation in radiosensitivity. Using an in vitro system, we conducted microarray to identify a miRNA signature to assess radiosensitivity. The data were validated by analyzing available Head and Neck Squamous Cell Carcinoma (HNSCC) samples in the cancer genome atlas (TCGA) database. A total of 27 miRNAs showed differential alteration in response to IR in an Ataxia-Telangiectasia Mutated (ATM) kinase-dependent manner. We validated the list and identified a five miRNA signature that can predict radiation responsiveness in HNSCC. Furthermore, we found that the expression level of ATM in these patients was correlated with the radiation responsiveness. Together, we demonstrate the feasibility of using a miRNA signature to predict the clinical responsiveness of HNSCC radiotherapy.
Source: Oncotarget
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2212349581