Work

A simplified Protocol to Induce Callogenesis in Protoplasts of Date Palm (Phoenix dactylifera L.) Cultivars

Year: 2015

Type: article

Abstract: BACKGROUND: In Algeria, date palm is currently confronted to the Bayoud disease. Biotechnological tools such as protoplastsfusion can appear as an alternative to ensure rapid multiplication and improvement of this species. OBJECTIVES: Callogenesis induction in protoplasts isolated from embryogenic callus of three date palm cultivars. MATERIALS AND METHODS: L.) cultivars (Deglet Nour, Akerbouch and Degla Beida) were studied. Protoplasts of each cultivar were cultured on a semi-solid medium supplemented with various hormonal balances. RESULTS: BAP allowed good viable protoplast maintenance as well as cell wall regeneration. After more than two months of culture, cell divisions were still occurring and microcalli became visible to the naked eye, containing a large number of cells. CONCLUSIONS: The developed protocol can be useful for application of somatic hybridization to improve date palm cultivars.

Source: Iranian Journal of Biotechnology

DOI: 10.15171/ijb.1054